Recent News about Nick
Nicholas Apostolopoulos
Research Assistant
B. A. in Molecular Biology and Biochemistry 2011, Rutgers University
RESEARCH

My work currently focuses on further developing and optimizing Oligopainting, which is a high-resolution, high-throughput method for visualizing chromosome positioning by in situ fluorescent hybridization (FISH). My specific goals are to increase our current yields of Oligopaint probes while cutting costs. I am also interested in using Oligopaints in conjunction with super-resolution microscopy to elucidate chromosome organization and structure within the nucleus, using RNA and DNA FISH to investigate gene clustering and transcription centers within the nucleus, and looking at strand-specific DNA replication and segregation in different cell types, specifically in Bell nuclei1.

RESEARCH INTERESTS

Genetics, transcriptional regulation, technology development, virology, immunology, human genetics, and human disease

REFERENCE

1) Gostjeva EV, Thilly WG. Stem cell stages and the origins of colon cancer: a multidisciplinary perspective. Stem Cell Rev. 2005;1(3):243-51. Review. PubMed PMID: 17142861.

PUBLICATIONS

Regulated antisense transcription controls expression of cell-type-specific genes in yeast.
Gelfand B., Mead J., Bruning A., Apostolopoulos N., Tadigotla V., Nagaraj V., Sengupta A.M., Vershon A.K.
Mol Cell Biol. 2011 Apr;31(8):1701-9.